Review





Similar Products

peptide aa16 and its derivatives  (GenScript corporation)
90
GenScript corporation peptide aa16 and its derivatives
Peptide Aa16 And Its Derivatives, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pm40048797-54-4-8?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
peptide aa16 and its derivatives - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
recombinant histidine-tagged sars-cov-2 spike protein (aa16-1213, rp-87671)  (Thermo Fisher)
90
Thermo Fisher recombinant histidine-tagged sars-cov-2 spike protein (aa16-1213, rp-87671)
Recombinant Histidine Tagged Sars Cov 2 Spike Protein (Aa16 1213, Rp 87671), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc09671159-163-2-11?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
recombinant histidine-tagged sars-cov-2 spike protein (aa16-1213, rp-87671) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
s1 (aa16-685) (wuhan)  (ACROBiosystems)
90
ACROBiosystems s1 (aa16-685) (wuhan)
S1 (Aa16 685) (Wuhan), supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc09461429__42003_2022_3866_MOESM1_ESM-6-28-32?v=ACROBiosystems
Average 90 stars, based on 1 article reviews
s1 (aa16-685) (wuhan) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sars-cov-2 orf8 (aa16-121), his tag rp-87666  (Thermo Fisher)
90
Thermo Fisher sars-cov-2 orf8 (aa16-121), his tag rp-87666
<t>ORF8</t> binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) <t>PBMCs</t> from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.
Sars Cov 2 Orf8 (Aa16 121), His Tag Rp 87666, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc09230529-85-10-17?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
sars-cov-2 orf8 (aa16-121), his tag rp-87666 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
orf8 (sars-cov-2 orf8 (aa16-121), his tag  (Thermo Fisher)
90
Thermo Fisher orf8 (sars-cov-2 orf8 (aa16-121), his tag
<t>ORF8</t> binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) <t>PBMCs</t> from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.
Orf8 (Sars Cov 2 Orf8 (Aa16 121), His Tag, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc09230529-77-13-15?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
orf8 (sars-cov-2 orf8 (aa16-121), his tag - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
orf8 protein (sars-cov-2 orf8 (aa16-121), his tag rp-87666  (Thermo Fisher)
90
Thermo Fisher orf8 protein (sars-cov-2 orf8 (aa16-121), his tag rp-87666
<t>ORF8</t> binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) <t>PBMCs</t> from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.
Orf8 Protein (Sars Cov 2 Orf8 (Aa16 121), His Tag Rp 87666, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc09230529-64-8-10?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
orf8 protein (sars-cov-2 orf8 (aa16-121), his tag rp-87666 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hexadecyl acrylate aa16  (Macklin Inc)
90
Macklin Inc hexadecyl acrylate aa16
<t>ORF8</t> binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) <t>PBMCs</t> from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.
Hexadecyl Acrylate Aa16, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/10__1016_slash_j__cej__2022__134851-55-0-15?v=Macklin+Inc
Average 90 stars, based on 1 article reviews
hexadecyl acrylate aa16 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sars-cov2 spike protein (s1 domain aa16-685, cat# z03485)  (GenScript corporation)
90
GenScript corporation sars-cov2 spike protein (s1 domain aa16-685, cat# z03485)
<t>ORF8</t> binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) <t>PBMCs</t> from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.
Sars Cov2 Spike Protein (S1 Domain Aa16 685, Cat# Z03485), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc07737878-28-0-8?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
sars-cov2 spike protein (s1 domain aa16-685, cat# z03485) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sars-cov2 spike protein (100 s1 domain aa16–685, cat# z03485)  (GenScript corporation)
90
GenScript corporation sars-cov2 spike protein (100 s1 domain aa16–685, cat# z03485)
<t>ORF8</t> binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) <t>PBMCs</t> from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.
Sars Cov2 Spike Protein (100 S1 Domain Aa16–685, Cat# Z03485), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aa16/pmc07523098-215-0-10?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
sars-cov2 spike protein (100 s1 domain aa16–685, cat# z03485) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


ORF8 binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) PBMCs from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.

Journal: Viruses

Article Title: SARS-CoV-2 Accessory Protein ORF8 Decreases Antibody-Dependent Cellular Cytotoxicity

doi: 10.3390/v14061237

Figure Lengend Snippet: ORF8 binds monocytes and NK cells through CD16a. ( A ) PMBCs were incubated with or without FITC-conjugated recombinant ORF8 protein on ice for 30 min, followed by staining with anti-CD14-V450, anti-CD16-PE-CY7 and anti-CD56-PE antibodies before being fixed with 4% PFA and analyzed by flow cytometry. CD3+ T cells were labeled with an anti-CD3-PE antibody. ( B ) AR2G biosensors loaded with CD16a protein were soaked in two-fold dilution series of ORF8 (31.25 nM–500 nM). Raw data are shown in blue and model in red . The dissociation/affinity constant (K D ), on rates (K a ) and off rates (K dis ) were calculated using a 1:1 binding model. ( C ) HEK293T cells were transfected with CD16a and ORF8 plasmid DNA, followed by co-immunoprecipitation with an anti-Flag antibody. The presence of ORF8 was detected with the anti-StrepII antibody. ( D , E ) PBMCs from different donors were thawed and incubated for 16 h with media from ORF8 DNA-transfected HEK293T cells. Alternatively, PBMCs were treated with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid. The following day, the PBMCs were stained with anti-CD3, anti-CD14, anti-CD19, anti-CD56, anti-CD16 and LIVE/DEAD Fixable Aqua Dead Cell Stain and analyzed by flow cytometry to measure surface levels of CD16 on ( D ) monocytes ( n = 5) and ( E ) NK cells ( n = 4). Cell-surface CD16 levels in presence of ORF8 were normalized on cell-surface CD16 detected in absence of ORF8. Statistical significance was evaluated using a parametric paired t -test. *, p < 0.05; ***, p < 0.001.

Article Snippet: Alternatively, PBMCs were treated with ORF8 at 50 ng/10 6 PBMCs (SARS-CoV-2 ORF8 (aa16-121), His Tag (RP-87666), Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Incubation, Recombinant, Staining, Flow Cytometry, Labeling, Binding Assay, Transfection, Plasmid Preparation, Immunoprecipitation, Control

ORF8 decreases PBMC-mediated ADCC. ( A ) Secretion of ORF8 into culture supernatant of HEK293T cells transfected with ORF8 DNA. ORF8 was probed with an anti-ORF8 antibody. ( B ) ADCC (%) mediated by plasma from 13 convalescent individuals, ( C ) 13 vaccinated individuals, and ( D ) 13 previously-infected vaccinated individuals using PBMCs from healthy donors as effector cells. ( E ) ADCC (%) mediated by plasma from convalescent individuals with PBMCs or monocytes as effector cells. The effector cells were treated overnight (16 h) with media from ORF8 DNA-transfected HEK293T cells (+ORF8) or with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid (−ORF8). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Viruses

Article Title: SARS-CoV-2 Accessory Protein ORF8 Decreases Antibody-Dependent Cellular Cytotoxicity

doi: 10.3390/v14061237

Figure Lengend Snippet: ORF8 decreases PBMC-mediated ADCC. ( A ) Secretion of ORF8 into culture supernatant of HEK293T cells transfected with ORF8 DNA. ORF8 was probed with an anti-ORF8 antibody. ( B ) ADCC (%) mediated by plasma from 13 convalescent individuals, ( C ) 13 vaccinated individuals, and ( D ) 13 previously-infected vaccinated individuals using PBMCs from healthy donors as effector cells. ( E ) ADCC (%) mediated by plasma from convalescent individuals with PBMCs or monocytes as effector cells. The effector cells were treated overnight (16 h) with media from ORF8 DNA-transfected HEK293T cells (+ORF8) or with an identical volume of conditioned media collected from HEK293T cells transfected with a control plasmid (−ORF8). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Alternatively, PBMCs were treated with ORF8 at 50 ng/10 6 PBMCs (SARS-CoV-2 ORF8 (aa16-121), His Tag (RP-87666), Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Transfection, Clinical Proteomics, Infection, Control, Plasmid Preparation